TTYH2 | Dark Matter

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCAGGCGGCGCGCGTGGACTACATCGCTCCCTGGTGGGTCGTGTGGCTGCACAGCGTCCCGCACGTCGGCCTGCGCCT
GCAGCCCGTGAACAGCACCTTCAGCCCCGGCGACGAGAGTTACCAGGAGTCGCTGCTGTTCCTGGGGCTGGTGGCCGCCG
TCTGCCTGGGCCTGAACCTCATCTTCCTTGTGGCTTACCTGGTCTGTGCATGCCACTGCCGGCGGGACGATGCGGTGCAG
ACCAAGCAGCACCACTCCTGCTGCATCACCTGGACGGCCGTGGTGGCCGGGCTCATCTGCTGTGCTGCGGTGGGCGTTGG
TTTCTATGGAAACAGCGAGACCAACGATGGGGCGTACCAGCTGATGTACTCCTTGGACGATGCCAACCACACCTTCTCTG
GGATCGATGCTCTGGTTTCCGGAACTACCCAGAAGATGAAGGTGGACCTAGAGCAGCACCTGGCCCGGCTCAGTGAGATC
TTTGCTGCCCGGGGCGATTACCTGCAGACCCTGAAGTTCATACAGCAGATGGCGGGCAGCGTTGTTGTTCAGCTCTCAGG
ACTGCCCGTGTGGAGGGAGGTCACCATGGAGCTGACCAAGCTATCCGACCAGACTGGCTACGTGGAGTACTACAGGTGGC
TCTCCTACCTCCTGCTCTTTATCCTGGACCTGGTCATCTGCCTCATTGCCTGCCTGGGACTGGCCAAGCGCTCCAAGTGT
CTCCTGGCCTCGATGCTGTGCTGTGGGGCACTGAGCCTGCTCCTCAGTTGGGCATCCCTGGCCGCTGATGGCTCTGCGGC
AGTGGCCACCAGTGACTTCTGTGTGGCTCCTGACACCTTCATCCTGAACGTCACGGAGGGCCAGATCAGCACAGAGGTGA
CTCGCTACTACCTGTATTGCAGCCAGAGTGGAAGCAGCCCCTTCCAGCAGACCCTGACCACCTTCCAGCGCGCACTTACC
ACCATGCAGATCCAGGTCGCGGGGCTGCTGCAGTTTGCCGTGCCCCTCTTCTCCACTGCAGAGGAAGACCTGCTTGCAAT
CCAGCTCCTGCTGAACTCCTCAGAGTCCAGCCTTCACCAGCTGACCGCCATGGTGGACTGCCGAGGGCTGCACAAGGATT
ATCTGGACGCTCTTGCTGGCATCTGCTACGACGGCCTCCAGGGCTTGCTGTACCTTGGCCTCTTCTCCTTCCTGGCCGCC
CTCGCCTTCTCCACCATGATCTGTGCAGGGCCAAGGGCCTGGAAGCACTTCACCACCAGAAACAGAGACTACGATGACAT
TGATGATGATGACCCCTTTAACCCCCAAGCCTGGCGCATGGCGGCTCACAGTCCCCCGAGGGGACAGCTTCACAGCTTCT
GCAGCTACAGCAGTGGCCTGGGAAGTCAGACCAGCCTGCAGCCCCCGGCCCAGACCATCTCCAACGCCCCTGTCTCCGAG
TACATGAACCAAGCCATGCTCTTTGGTAGGAACCCACGCTACGAGAACGTGCCACTAATCGGGAGAGCCTCCCCTCCGCC
TACGTACTCTCCCAGCATGAGAGCCACCTACCTGTCTGTGGCGGATGAGCACCTGAGGCACTACGGGAATCAGTTTCCAG
CCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG