Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGTTTGATAATGTCAGTGCCAGCAAACGAGAAGAGATCCTAGCTGGGTTTCCATGTTGCAGATCAGCTCCTTCTGACGT
GATCCTAGTAAGAGATGAAATAGCTCTTCTGCACAGATACCAGTCACAGATGCTGGGTTGGATTTTGATCACCTTGGCAA
CCATTGCTGCCTTAGTCTCCTGCTGTGTGGCAAAGTGCTGCTCTCCCCTCACCTCTCTGCAACATTGCTACTGGACCAGC
CACCTCCAGAATGAGAGAGAACTCTTTGAACAAGCAGCAGAGCAGCACTCTCGGCTCCTCATGATGCATCGCATAAAGAA
GCTATTTGGCTTCATTCCCGGGAGTGAAGACGTCAAACACATCCGCATTCCTTCTTGTCAGGACTGGAAAGATATTTCAG
TACCCACTCTTTTATGCATGGGTGATGACTTGCAAGGTCACTATAGCTTCCTTGGAAATAGGGTGGATGAGGATAATGAG
GAAGACAGATCAAGAGGTATTGAATTAAAACCTACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCT
CCTCGGTCTCGATTCTACGTAG