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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-LRRC55-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA
CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC
CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC
TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC
AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC
TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC
TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT
GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT
ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC
TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT
GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC
TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC
CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_LRRC55_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA
CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC
CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC
TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC
AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC
TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC
TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT
GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT
ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC
TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT
GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC
TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC
CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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