10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for LRRC55 Gene
10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-LRRC55-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single cell clone: D5
Subcellular localization: Intracellular + perinuclear
Sequence
ORF Sequence:
ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_LRRC55_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA
CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC
CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC
TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC
AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC
TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC
TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT
GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT
ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC
TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT
GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC
TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC
CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC
CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC
TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC
AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC
TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC
TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT
GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT
ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC
TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT
GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC
TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC
CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Snapgene File:
Basic Information
Gene Taxonomy:
Construct ID:
IDG_LRRC55_UE_1
Virus Type:
Selection:
Puro,Amp
Vector:
783_hU6_EGFP
Passed QC:
On
Vector Type:
Sequence
Inserted_sequence:
GCCCAGGTGTCACCCATCTT
Media and Files
Vector Map:
Snapgene File: